第三屆 Igenomix 亞太區線上學術論壇

Abstract

Objective: The aim of this study is to clarify the efficacy of ERA leading to personalization of the day of embryo transfer in RIF patients.
Methods: A retrospective study was performed for 94 patients with RIF who underwent ERA between July 2015 and December 2019. Pregnancy outcomes in a previous vitrified-warmed blastocyst transfer (previous VBT) and a personalized vitrified-warmed blastocyst transfer (pVBT) in identical patients were compared. The details of each pVBT were further analyzed between patients in a non-displaced group, which indicated “receptive” cases in ERA results and those who were in the displaced group, which indicated “non-receptive” cases.
Results: When the pregnancy rate, both per patient and per transfer cycle, of previous VBT and pVBT were compared, a significant increase in pVBT was observed between the two methods. The pregnancy rates, implantation rates, and clinical pregnancy rates of the first pVBT were significantly higher in the displaced group than the non-displaced group. The cumulative ongoing pregnancy rate of the displaced group tended to be higher compared to that of the non-displaced group in the first pVBT.
Conclusions: Our study demonstrates that pVBT guided by ERA tests may improve pregnancy outcomes in RIF patients whose WOI is displaced.

Biography

A graduate of Jikei University School of Medicine, he has trained extensively throughout Jikei University Hospital, Keiai Reproductive & Endosurgical Clinic, and Kyono ART Clinic Takanawa. He is currently on a staff at Kyono ART Clinic Takanawa and is also engaged in research on reproductive endocrinology as a graduate student at Jikei University School of Medicine. He is Board Certified Fellow of Japan Society of Obstetrics and Gynecology, and Japan Society for Reproductive Medicine.

Awards / Publications

1. Kasahara Y., Hashimoto T., Yokomizo R., Takeshige Y., Yoshinaga K., Toya M., Igarashi H., Kishi H., Kyono K.. Evaluation of Pregnancy Outcomes of Vitrified-Warmed Blastocyst Transfer before and after Endometrial Receptivity Analysis in Identical Patients with Recurrent Implantation Failure. FERTILITY & REPRODUCTION. 2021; 3: 1-7
2. Kasahara Y., Yokomizo R., Ueda K., Makishima R., Kishi H., Okamoto A.. Analysis of Surgical Procedure of Four Cases of Ovarian Pregnancies Treated with Laparoscopic Surgery. Gynecol Minim Invasive Ther. 2021;10(2):117-120
3. Hosoya S., Kasahara Y., Komazaki H., Kishi H., Takano H., Okamoto A.. A rare case of heterotopic pregnancy after a single embryo transfer: A case report and literature review. J Obstet Gynaecol Res. 2021 Jul 12.

Abstract

Implantation is a complex process that requires synchronous and cross-talk between a healthy embryo and a receptive and microbiome composition endometrium. The aim of this study was to compare clinical outcomes in patients of recurrent implantation failure (RIF), who had embryo transfer following the EndomeTRIO testing and a personalized embryo transfers. The study was approved by the Institutional Review Board of Chung Shan Medical University Hospital, Taichung, Taiwan (CS-18191). This study included 93 infertility patients who have been advised ERA test and/or admitted to Lee Women’s Hospital, between November 2018 and May 2021. For a mock cycle prior their frozen blastocyst transfer cycle that included an endometrial biopsy for the EndomeTRIO testing. The clinical pregnancy rate of RIF patients with pET after non- receptive ERA was higher than the receptive ERA group (71.2% vs 51.2%). The clinical pregnancy rate after treating abnormal Endometrial Microbiome Metagenomic Analysis group was 80% (16/20). Performing the EndomeTRIO testing test increased the clinical pregnancy rate in RIF patients who were non-receptive implantation or exiting abnormal Microbiome endometrium. Further large prospective studies are needed to elucidate the role of EndomeTRIO testing testing in patients with recurrent implantation failure.

Biography

Dr Chun-I Lee was awarded an MD in 2008 from the National Defense Medical Center and completed his clinical training in reproductive medicine in 2016 in National Taiwan University College Medicine and his PhD from the Institute of Medicine, Chung Shan Medical University. Dr. Chun-I Lee professes on the Endocrine and Infertility medicine and Obstetrics and Gynecology. He is the CEO in Lee Women’s Hospital. He has worked with infertile couples for over 5 years and has achieved lots of healthy babies after treatment of couples by IVF. He has performed and published many studies on clinical reproductive medicine.

Awards / Publications

1. The third Prize in the Oral Research Paper Publication at the Annual Meeting of Taiwanese Society for Reproductive Medicine Research Paper in 2019　(Title: Potential of Using AI Artificial Intelligence Test Tubes to Improve the Pregnancy Rate)
2. Lee CI, Cheng EH,  Lee MS, Lin PY, Chen YC, Chen CH, Huang LS, Huang CC,  Lee TH. Healthy live births from transfer of low-mosaicism embryos after preimplantation genetic testing for aneuploidy. J Assist Reprod Genet. 2020 Sep;37(9):2305-2313.
3. Lee CI, Chen HH, Huang CC, Lin PY,  Lee TH,  Lee MS. Early Progesterone Change Associated With Pregnancy Outcome After Fresh Embryo Transfer in Assisted Reproduction Technology Cycles With Progesterone Level of >1.5 ng/ml on Human Chorionic Gonadotropin Trigger Day. Front Endocrinol (Lausanne). 2020 Sep 15;11:653.
4. Lee CI, Su YR, Chen CH, Chang TA, Kuo EE, Zheng WL, Hsieh WT, Huang CC, Lee MS, Liu M. End-to-end deep learning for recognition of ploidy status using time-lapse videos. J Assist Reprod Genet. 2021 May 22. Online ahead of print.
5. Lee CI, Chen HH, Huang CC, Chen CH, Cheng EH, Huang JY, Lee MS, Lee TH. Effect of Interval between Human Chorionic Gonadotropin Priming and Ovum Pick-up on the Euploid Probabilities of Blastocyst. J Clin Med. 2020 Jun 2;9(6):1685.

Abstract

Whether ERA can effectively improve embryo implantation and clinical pregnancy rate in patients with RIF has been controversial. Through retrospective analysis, RIF patients were divided into experimental group (era-RIF) and control group (RIF). The implantation rate and clinical pregnancy rate after embryo transfer between the two groups were compared to determine whether ERA detection is effective for RIF patients.

Biography

• Deputy Chief Physician, Doctor of Reproductive Medicine of Sun Yat-Sen University, master supervisor
• Director for Liuzhou Institute of Reproduction and Genetics
• Deputy Director for Reproductive Center, Liuzhou Maternal and Child Health Hospital
• Member of the Reproductive Health Committee of the Chinese Society of Rehabilitation Medicine Institute
• Member of the Reproductive Medicine Professional Committee of Guangxi Medical Doctor Association
• She worked in IVF Australia for further learing and attended Shanghai Renji Hospital to study reproductive immunity. She is proficient in the treatment of repeated implantation failures and recurrent miscarriage.
• Presided 1 project which is funded by National Natural Science Foundation of China, and another one supported by Guangxi Natural Science Foundation. 2 research projects are include in the Liuzhou Science and Technology Research and Technology Development Program, and she published 5 SCI papers as the first author.

Awards / Publications

1. Ma W, Huang X, Yang X, Liang X*. Maternal obesity in mice not only affects fresh embryo quality but also aggravates injury due to vitrification, J Assist Reprod Genet, 2016, 33(11), 1515-1523.
2. Wenhong Ma*, Lihong Wei, Ni Tang, Jihong Wei. Effects of a low hCG dose of 2000 IU on clinical outcomes in high-responder women undergoing IVF/ICSI, Int J Clin Exp Med, 2016, 9(7), 14677-14683
3. Wei L-H, Ma W-H, Tang N, Wei J-H*. Luteal-phase ovarian stimulation is a feasible method for poor ovarian responders undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer treatment compared to a GnRH antagonist protocol: A retrospective study, Taiwanese Journal of Obstetrics & Gynecology, 2016, 55(1), 50-54
4. Ma W, Zhan Z, Liang X*, Chen J, Huang X, Liao C. Subclinical impairment of ovarian reserve in systemic lupus erythematosus patients with normal menstruation not using alkylating therapy. J Womens Health (Larchmt), 2013, 22(12),1023-1027
5. Ma W, Yang X, Liang X*. Obesity does not aggravate vitrification injury in mouse embryos: a prospective study. Reprod Biol Endocrinol, 2012,10(1), 68-73
6. 马文红,张玉,李忻琳,韦立红,唐妮.女性单纯超重或肥胖对新鲜移植周期胚胎质量及妊娠结局的影响[J].广西医学,2021,43(02):142-145+159.
7. 马文红,张玉,李忻琳*,韦立红,唐妮.超重及肥胖对胚胎玻璃化冷冻损伤的影响[J].实用医学杂志,2020,36(15):2077-2082.
8. 韦立红,马文红*,李忻琳, 等.卵泡期联合黄体期促排卵对卵巢低反应患者活产率的影响[J].生殖医学杂志,2019,28(1):36-40.
9. 马文红*,韦立红,李忻琳, 等. hCG剂量与IVF/ICSI高反应患者获卵率的相关性[J].中国优生与遗传杂志,2017,25(6):101-104.
10. 吴秀芬,马文红*.中孕期宫颈功能不全患者施行紧急宫颈环扎术的临床分析[J].中华妇幼临床医学杂志（电子版）,2016,12(6):686-689.
11. 马文红，韦立红，唐妮，韦继红*，在IVF/ICSI周期中正常反应患者hCG剂量与获卵率的相关性，实用医学杂志，2015，31（24）：4062 ～ 4065
12. 马文红，韦继红*，自然周期黄体生成激素峰诱发卵子成熟的分子机制，国际生殖健康/ 计划生育杂志，2015，34 ( 4 )，333～337
13. 马文红，梁晓燕*，人绒毛膜促性腺激素诱发排卵的优缺点，生殖医学杂志，2012，21（6）： 547 ～ 550
14. 马文红，梁晓燕*，各种控制性卵巢刺激方案中诱发排卵的特点及运用，国际生殖健康/计划生育杂志， 2012，31（5）：261 ～ 264
15. 马文红，梁晓燕*，多囊卵巢综合征控制性卵巢刺激的有效运用，国际生殖健康/计划生育杂志，2011，30（5）：375 ～ 379
16. 梁晓燕*，马文红，杨刚，控制性卵巢刺激中HCG注射后雌二醇浓度下降的原因分析，中山大学学报(医学科学版)，2010，31（2）：530 ～ 534

Abstract

Many researchers have reported that asynchronous coordination between embryonic development and endometrial receptivity is the crucial cause of implantation failure, and the importance of finding the optimal and personalized endometrial receptivity time to improve implantation is emphasized.
In addition, research and the application of molecular evaluation are gradually developing to find means of a healthier pregnancy by checking the status of the endometrium in various aspects, such as microbiomes.
In this presentation, the endometria of patients with failed implantation were diagnosed using endometrial receptivity array (ERA) and histological assessment and analyzed according to clinical values such as biopsy time, age, number of implantation failures, and endometrial thickness. On that basis, we would like to share our recent experience with the personalized embryo transfer (ET) of blastocysts and embryos at the cleavage stage based on molecular diagnosis.

Biography

Education and professional positions held

• Ph.D, Biochemistry, Hanyang University
• 2011-present: Science and Laboratory Director, Fertility Research Institute, Seoul Rachel Fertility Center
• 1989-2010: Chief Research Scientist, CHA Fertility Center of CHA General Hospital

Board and members of societies

• 2011-present: Chief Research Scientist, Korean Association for clinical embryologist
• 2016-present: Director, Policy Development Committee: Korean Society of Developmental Biology
• 2018- present: Expert in technology level evaluation, Ministry of science and ICT, Korea
• 2019-2020: President, Korean Association for clinical embryologist

Publications

1. Ryu MJ, Seo BJ, Choi YJ, Han MJ, Choi Y, Chung MK, Do JT. Mitochondrial and metabolic dynamics of endometrial stromal cells During the Endometrial Cycle. Stem Cells Dev. 2020 Sep 24.
2. Sohn JO, Seong SY, Kim HJ, Jo YM, Lee KH, Chung MK, Song HJ, Park KS, Lim JM. Alterations in intracellular Ca2+ levels in human endometrial stromal cells after decidualization. Biochem Biophys Res Commun. 2019 Jul 23;515(2):318-324.
3. Chung MK, Jeong HJ, Lee JH, Park SJ, Chung HD, Kang HY. Comprehensive chromosome analysis of blastocysts before implantation using array CGH. Mol Cytogenet. 2013 Jun 3;6(1):22.
4. Kim SS, Lee WS, Chung MK, Lee HC, Lee HH, Hill D. Long-term ovarian function and fertility after heterotopic autotransplantation of cryobanked human ovarian tissue: 8-year experience in cancer patients. Fertil Steril. 2009 91(6):2349-54.
5. Cha KY, Chung HM, Lee DR, Kwon H, Chung MK, Park LS, Choi DH, Yoon TK. Obstetric outcome of patients with polycystic ovary syndrome treated by in vitro maturation and in vitro fertilization-embryo transfer. Fertil Steril. 2005 May;83(5):1461-5.

Awards

1. The Best Poster Presentation Award, the annual meeting of the PSRM, 2018
2. The Best Poster Presentation Award, the annual meeting of the KSRM, 2003, 2005,2015, 2019
3. The SART Prize Paper, the annual meeting of the ASRM, 2007
4. The best prize paper, the annual meeting of the KSRM, 2006

Abstract

Recurrent implantation failures (RIF) are challenging all IVF teams and remain devastating for patients with infertility. Guidelines to perform exams have been provided after twice or three times of embryo transfers without pregnancy. However, a large part of these failures remains unexplained.
In this study, we present our strategy of a thorough investigation to understand the origin of these failures. Our results demonstrate that an individualized and multidisciplinary strategy should be adopted to improve implantation rate and live birth rate for couples with RIF.

Biography

Dr. Chien has more than 10 years of experience in Obstetrics and Gynecology, and specialized in Assisted Reproductive Technology Field. Dr. Chien graduated from University of Medical Sciences of Havana for his MD and then post-graduated with Excellent Degree.
Dr. Chien is now working mainly in the treatment for infertility couple, from basic techniques such as: control of ovarian stimulation, perform egg retrieval, Embryo transfer… to advanced techniques: finding and treating reasons of infertility, performing basic and advanced techniques in Obstetrics and Gynecology.
He is also one of the pioneers in using Endometrial Receptivity Analysis in Vietnam and has succeed in helping his patients delivering healthy babies.

EXPERIENCE

• 08/2021 – Now: Head of Department, A.R.T Center, Vinmec Times City International Hospital
• 2020 – 07/2021: Deputy Head of Department, A.R.T Center, Vinmec Times City International Hospital
• 2013 – 2020: Clinical Physician, A.R.T Center, Vinmec Times City International Hospital
• 2009 – 2012: Clinical Physician, Hospital Gineco-Obstétrico América Arias

Awards / Publications

• Completed a study to assess the role of 3D gynecological ultrasound in diagnosing intrauterine problems causing infertility
• Started a study about using stem cells to treat severe intrauterine cavity adhesion

Abstract

Recurrent implantation failure is the most frustrating and distressing situation for patients and their treating doctor alike. This presentation will present a few cases where assessing the endometrial receptivity of the endometrium has been performed, and will discuss the indication for this invasive but often illuminating assessment.

Biography

Professor Roger Hart is sub-specialist in reproductive endocrinology and infertility (CREI), he is the Medical Director of Fertility Specialists of Western Australia and Professor of Reproductive Medicine, Deputy Head of the Division of Obstetrics and Gynaecology at University of Western Australia, and the lead clinician for the public fertility service of Western Australia. He has active research interests in the early life origins of male and female reproductive disorder, polycystic ovary syndrome, the longer-term outcomes after IVF treatment, and all aspects of infertility management, and has a particular interest in fertility preservation for patients with a cancer diagnosis. He has published over 200 chapters and research papers in the field of reproductive medicine.

Awards / Publications

1. Hart R. Physiological Aspects of Female Fertility: The Role of the Environment, Modern Lifestyles and Genetics Physiological Reviews 2016 Jul;96(3):873-909
2. Hart RJ, Doherty DA, Keelan JA, McLachlan R, Skakkebaek NE, Norman RJ, Dickinson JE, Pennell CE, Newnham JP, Hickey M, Handelsman DJ. Early life events predict adult testicular function; data derived from the Western Australian (Raine) birth cohort. J Clin Endocrinol Metab. 2016; 101(9):3333-44.
3. Hart R, Doherty DA ,McLachlan R, Walls ML, Keelan JA, Dickinson J, Skakkebæk NE, Norman RJ, Handelsman DJ. Testicular Function in a Birth Cohort of Young Men. Human Reproduction 2015 ;30(12):2713-24
4. Hart R, Doherty D. The Potential Implications of a PCOS Diagnosis on a Woman’s Long-term Health Using Data Linkage. J Clin Endocrinol Metab 2015 Mar;100(3):911-9.
5. Hart RJ, Doherty DA, Mori TA, Adams LA, Huang R-C, Minaee N, Handelsman DJ, R McLachlan R, Norman RJ, Dickinson JE, Olynyk JK, Beilin LJ. Features of the metabolic syndrome in late adolescence are associated with impaired testicular function at 20 years of age. Human Reproduction (DOI: 10.3410/f.734674925.793571368) 2019.